Product Protective Capture and Elution of Biopharmaceutical Agents
// Cell Lines // Life Sciences // Red Biotechnology // White Biotechnology
Ref-Nr: 15771
Abstract
The field of application of this product-sparing affinity chromatography lies in the field of Life Science / White Biotechnology / Cell Lines and Medicine / Pharmaceuticals. It enables the direct processing of biopharmaceutical active substance molecules from cell culture supernatants with the help of ligands of special quality (see also 16265 / InnoLand). Biotechnologically produced active substances are usually worked up by multistage chromatographic separation processes as shown in the figure below (see Fig. 1). By affinity-chromatic process steps, high purification factors can be achieved with low yield losses.
background
The biotechnological production of biopharmaceutical active substances using multi-stage chromatographic separation processes should be as gentle on the product as possible and cost-effective.
Innovation / Solution
The invention describes a process that enables the gentle affinity purification of biopharmaceuticals at neutral pH. Finally, the bound glycoproteins are released at neutral pH with an elution buffer containing a competitively binding substance. The invention also describes simple new multivalent ligands with significantly higher specificity and selectivity than previous monovalent boronates. This enables the selective purification of biopharmaceuticals that lose therapeutic activity and product quality due to acid elution.Benefits
Containment of manufacturability risk
Monitoring effort and in-process controls can be saved
efficient removal of product- and prodcess-related impurities at the very first downstream step
Non-immunologic ligand - less effort for leachables monitoring
Possible further applications for dedicated virus removal.
fields of application
The invention enables the direct processing of biopharmaceutical active substance molecules from cell culture supernatant by means of low-cost affinity chromatography, which is particularly gentle on the product.You can close this window. You can find your search results in the previous window
